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Department of Entomology, S.V. Agricultural College, ANGRAU, Tirupati-517 502.
The objective of the investigation is to study the diversity of Bacillus thuringiensis and actinomycetes in groundnut field during kharif 2022 after pesticide spray with different spray appliances viz., knapsack sprayer, power sprayer and drone sprayer. Foliar application of imidacloprid at 30 days after sowing and chlorantraniliprole + hexaconazole at 55 days after sowing was done against the insect pests and foliar disease control in groundnut. The soil samples were collected at the time of sowing, 45 DAS and at harvest from the different treatments to study the impact or effect of different treatments on soil microbes population. The treatments were 1) Chemical sprayed with knapsack sprayer, 2) Chemical sprayed with power sprayer, 3) Chemical sprayed with drone spray with 75% RDP, 4) Chemical sprayed with drone spray with 100% RDP and 5) Chemical sprayed with 50% RDP, 6) Untreated control. The microbial organisms isolated and identified through standard microbial procedures for Bacillus thuringiesis and actinomycetes. The results indicated that, the number of CFUs obtained for the two organisms i.e., thuringiensis and actinomycetes from soil samples of different treatments at the time of sowing, 45 DAS and at harvest did not vary significantly. Hence, it can be concluded that, there was no significant influence of the drone spray, power spray and knapsack spray on population fluctuation of B. thuringiensis and actinomycetes in groundnut crop after one season of evaluation.
KEYWORDS: Actinomycetes, Bacillus thuringiensis, Drone spray, Groundnut insect pests, Isolation from soil.