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S.J. MALLIKARJUNA*, M. HEMANTHKUMAR, D.M. REDDY, P. SUDHAKAR, M. PARAMESH AND N.P. ESWARA REDDY
Department of Genetics and Plant Breeding, S.V. Agricultural College, Tirupati-517 502, Andhra Pradesh, India
Studies were carried out to establish an efficient system for callus induction in two sugarcane clones (2008T42 and 2009T5) during 2012. Leaf rolls were used as explants for callus induction. Two growth regulators, 2, 4-D and BAP with different concentrations (1-5 mg l-1) were used for callus induction. Cultures were placed in 24 hours dark at 24 ± 2°C with 70% relative humidity. The two sugarcane clones (2008T42 and 2009T5) exhibited better response for callus induction in 2, 4-D at 4 mg l-1 with minimum number of days taken for callus initiation (9.7 – 10.2), maximum number of explants inducing callus (6.00) and the highest callus induction frequency (100 per cent).
KEY WORDS: BAP, Callus induction, 2, 4-D, Leaf roll,